We narrowed to 18,336 results for: met

1. PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)

   Plasmid

   #174527

   Purpose

   Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Insert

   PylS
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pD864-LacZ backbone

   Plasmid

   #109384

   Purpose

   Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PHBA01

   Plasmid

   #83535

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC gene

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-UBiC-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCW-SPI1

   Plasmid

   #162824

   Purpose

   Doxycycline-inducible overexpression of human SPI1

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTrc-trGPPS(CO)-LS

   Plasmid

   #50603

   Purpose

   pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

   Depositor

   Jay Keasling

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Inserts

   Limonene Synthase
   ApR
   LacIq
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   truncated codon optimized geranylpyrophosphate sy…

   Promoter

   Trc

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPHT-PIP2

   Plasmid

   #60937

   Purpose

   Fluorescent FPX sensor for PIP2

   Depositor

   Anne Marie Quinn

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   PIP2 dimerization dependent sensor RA+B monomers
   

   Use

   Tags

   dimerization dependent fluorescent protein monome…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW-SPIC

   Plasmid

   #162825

   Purpose

   Doxycycline-inducible overexpression of human SPIC

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   SPIC (SPIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKA-207I10

   Plasmid

   #79845

   Purpose

   Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK-GFP

    Plasmid

    #66844

    Purpose

    Tet-inducible expression of GFP in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-HA-YFP-Raptor K840R

    Plasmid

    #204418

    Purpose

    Acetylation mutant for human raptor

    Depositor

    David Rubinsztein

    Article

    Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

    Insert

    Regulatory Associated Protein Of MTOR, Complex 1
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Lysine 840 to Arginine (K840R)

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-HA-YFP-Raptor K1097R

    Plasmid

    #204417

    Purpose

    Acetylation mutant for human raptor

    Depositor

    David Rubinsztein

    Article

    Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

    Insert

    Regulatory Associated Protein Of MTOR, Complex 1
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Lysine 1097 to Arginine (K1097R)

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQE30-mBFP

    Plasmid

    #112682

    Purpose

    Expression of NADPH-dependent blue fluorescent protein

    Depositor

    Geun-Joong Kim

    Article

    Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

    Insert

    His-mBFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. M3D395A-M14-dCas9

    Plasmid

    #134780

    Purpose

    Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D395A

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. groSL-GFP

    Plasmid

    #109389

    Purpose

    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pgroSL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. htpG1-GFP

    Plasmid

    #109387

    Purpose

    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG1-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ppst93-ANME2bHR1 MCR-NG

    Plasmid

    #192774

    Purpose

    Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge