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p667-UBC-NRF3-V5-miniTurbo

Plasmid

#207816

Purpose

C-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF3-V5-miniTurbo (NFE2L3 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro

Plasmid

#209644

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro

Plasmid

#209643

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (170-439)

Plasmid

#203430

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCbs Flag CMYC (bHLH/LZ)

Plasmid

#203424

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE1

Plasmid

#195367

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

Tags

3xflag

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Rat, Aquifex aeolicus)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot9_19-285_AF

Plasmid

#148733

Purpose

Bacterial Expression of HsNot9_19-285

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9_19-285 (CNOT9 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot9_18-285_S

Plasmid

#147480

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1535-1706_K

Plasmid

#146791

Purpose

Mammalian Expression of HsXRN1_1535-1706

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1535-1706 (XRN1 Human)

Expression

Mammalian

Mutation

one non silent mutation S1688P compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-TEV-HsRCK_296-472_E

Plasmid

#146209

Purpose

Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsRck_296-472 (DDX6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

Plasmid

#195865

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V

Plasmid

#147813

Purpose

Mammalian Expression of HsDCP2-F404AF407AF409A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-F404AF407AF409A (DCP2 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

doublecortin (DCX Human)

Tags

TurboID and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA5-mVenus

Plasmid

#185646

Purpose

NDUFA5 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA5 (NDUFA5 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc LbaCas12a

Plasmid

#182125

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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