We narrowed to 15,941 results for: nans
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Plasmid#47710PurposeExpresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP2
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-mStrawberry
Plasmid#187224PurposeExpresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseCRISPRTagsInserted T2A-mStrawberry at 3' terminal of M…ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-RFP
Plasmid#65448PurposeExpression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectorsDepositorInsertSV40-RFP
UseLentiviralExpressionMammalianPromoterUBQAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huBim_Ex3
Plasmid#85532PurposeInducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporterDepositorInserthu Bim
UseCRISPR and LentiviralExpressionMammalianPromoterH1tAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
Plasmid#105039PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-S473A
Plasmid#49190PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalMutationS473APromoterT7Available SinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) M-Ras(WT)
Plasmid#224287PurposeCell transfection and expression of M-RasDepositorInsertM-Ras (MRAS Human)
ExpressionMammalianAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
V5-bArr1
Plasmid#201491PurposeExpression of the beta-arrestin1 containing a N-terminal V5 tag.DepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W
Plasmid#105034PurposeLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-C1-centrin-1
Plasmid#72588PurposemEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1DepositorAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1
Plasmid#176537PurposeNon-protein coding locus components from KraIscB-1DepositorInsertnon-coding locus components from KraIscB-1 locus
ExpressionBacterialAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Fly, Synthetic)
TagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5B-DN-CMV-GFP
Plasmid#83262PurposeAdenoviral expression of dominant negative STAT5B with co-expression of GFPDepositorInsertsUseAdenoviralTagsFLAGMutationdominant negative (Y699F)PromoterCMVAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf40
Plasmid#12728PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf40
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHS1477
Plasmid#205970PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)DepositorInsertCas5-HNH all proteins, with His-tagged Cas11
Tags14xHis on Cas11ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
Plasmid#37086PurposeExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-YFP
UseAAV and Cre/Lox; Cre-offTagsEYFPPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only