We narrowed to 16,214 results for: IGH@

1. pRK5_tAKARα

   Plasmid

   #119913

   Purpose

   Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARα
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-Amp-T7-roGFP

   Plasmid

   #202464

   Purpose

   Expresses redox sensitive GFP (roGFP) with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLifeAct_mScarlet-H_N1

   Plasmid

   #85055

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Accessory plasmid 2

   Plasmid

   #128571

   Purpose

   for cloning identified individual SPECS

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAtetO_6xHis-v5.9

   Plasmid

   #155259

   Purpose

   Plasmid for the aTc-inducible expression of the v5.9

   Depositor

   Andrew Ellington

   Article

   Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

   Insert

   v5.9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tet PA

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_NR1D1_Hormone-recep

   Plasmid

   #109960

   Purpose

   Protein expression and purification of NR1D1_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   NR1D1_Hormone-recep
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pc3xTC20-alphaTubulin

   Plasmid

   #36327

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

   Insert

   alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
   

   Use

   Tags

   3xTC20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-DUET-1-mRhubarb720-HO

   Plasmid

   #141201

   Purpose

   Expresses mRhubarb720 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb720
   heme oxygenase
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, L196Q, T202D, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-TET2Puro-gal4DBD-miniVPR-HA

   Plasmid

   #207169

   Purpose

   Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   Gal4DBD-miniVPR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-UBC-EGFP-2A-BoNT-B (LC)

    Plasmid

    #185722

    Purpose

    Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    botulinum neurotoxin type B light chain
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-ALFA-tag-Gal4-VP16

    Plasmid

    #199465

    Purpose

    Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    Gal4-ALFA-tag-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mc2 blast

    Plasmid

    #206217

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-CaMKIIa-C1C2-TS-EYFP

    Plasmid

    #35520

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    Trafficking signal and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBP-CRY1-PHR

    Plasmid

    #26035

    Depositor

    Johann Deisenhofer

    Article

    Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    CRY1 (CRY1 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Plasmid

    #192123

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PAmCherry1-N1-PHPLCd1

    Plasmid

    #202679

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dendra2-LaminB1-10

    Plasmid

    #57728

    Purpose

    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_NR6A1_Hormone-recep

    Plasmid

    #109945

    Purpose

    Protein expression and purification of NR6A1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR6A1_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge