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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEb

Plasmid

#193832

Purpose

Express TadCBEb (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDb-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pHD-pYtag(Clover)-ScarlessDsRed

Plasmid

#244931

Purpose

Vector containing a Clover-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

Clover-tagged pYtag system (3xITAM P2A Clover-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

ChroME-ST

Use

AAV

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-GRK6

Plasmid

#32693

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK6 (GRK6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Keppel

Plasmid

#214444

Purpose

BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Plasmid

#214265

Purpose

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

Depositor

D. James Surmeier

Insert

Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

Synapsin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomArchon

Plasmid

#126943

Purpose

Cre-dependent expression of SomArchon under CAG promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS nErCas12an

Plasmid

#132642

Purpose

Used for in vitro transcription of dual NLS ErCas12a mRNA

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

CRISPR; In vitro transcription

Tags

SV40 NLS

Promoter

T3

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100845

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-JNK1a1

Plasmid

#47574

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

JNK1a1 (MAPK8 Human)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Venus-Actb

Plasmid

#169788

Purpose

An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MTNR1A-DuET

Plasmid

#213348

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MTNR1A (MTNR1A Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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