We narrowed to 12,356 results for: nsf

Showing: 7841 - 7860 of 12356 results

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pAAV Fos YFP MS2

Plasmid

#131775

Purpose

Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

AAV and CRISPR

Promoter

n/a

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-DreO-4x6T

Plasmid

#196413

Purpose

Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

DreO

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mCherry-WPRE

Plasmid

#202620

Purpose

Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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-4200GAMT-P1-pGL4

Plasmid

#26804

Depositor

Sam Lee

Article

Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

Insert

Guanidinoacetate N-methyltransferase (GAMT Human)

Use

Luciferase

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO {ChETA}on-WPRE

Plasmid

#111386

Purpose

AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Insert

ChETA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

humanized ChR2, E123T/H134R

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLTR PKC beta II

Plasmid

#8417

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-JEDI-1P-WPRE

Plasmid

#202616

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-SYN-GFP-BGHpA

Plasmid

#190229

Purpose

Expresses EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p-715MRPprom-MRPcucuc

Plasmid

#87199

Purpose

Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

RMRP (RMRP Human)

Expression

Mammalian

Mutation

GAGAG91-95 to CUCUC

Promoter

human RMRP

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209660

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-fCAMK-GFP

Plasmid

#22917

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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ScFv-2ERT2-V

Plasmid

#120553

Purpose

3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

scFvGCN4, sfGFP, GB1, ERT2, VP64

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H GC editing reporter

Plasmid

#198884

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGG)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NEUROD1-T2A-mCherry

Plasmid

#178584

Purpose

Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-mPRMT7-GFP

Plasmid

#87104

Purpose

Express mouse PRMT7 with Flag-tag in mammalian cells

Depositor

Min Gyu Lee

Article

Lee et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

Protein arginine methyltransferase 7 (Prmt7 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-TVA-mCherry

Plasmid

#149295

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Mutation

Start codon removed.

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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