We narrowed to 26,306 results for: Vit

1. pU6 SgRNA GAPDH

   Plasmid

   #162736

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for GAPDH
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCherry-TSC2(AA)

   Plasmid

   #192442

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCardinal-NUP50-N-10

   Plasmid

   #56170

   Purpose

   Localization: Nuclear Pore, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   NUP50 (NUP50 Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

   Plasmid

   #124546

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
   

   Expression

   Mammalian

   Mutation

   WT, WT

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRibo-APEX2m

   Plasmid

   #176842

   Purpose

   Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGIPz-PD-L1 3SA

   Plasmid

   #121490

   Purpose

   Expression of human PD-L1 3SA with PD-L1 shRNA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Inserts

   PD-L1 3SA
   humun PD-L1 shRNA
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S176A, T180A, S184A

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. C1-FKBP(F36V):mCherry-Akt2(K14A)

   Plasmid

   #72906

   Purpose

   Recruitable Akt2(K14A) mutant

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   RAC-beta serine/threonine-protein kinase (AKT2 Human)
   

   Tags

   FKBP(F36V), mCherry

   Expression

   Mammalian

   Mutation

   K14A mutation

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScalps ACE2 hygro

    Plasmid

    #184786

    Purpose

    Stable expression of Human Ace2

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-evoA1-seBEN_empty

    Plasmid

    #174701

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEN
    

    Use

    Lentiviral

    Tags

    myc tag

    Promoter

    EFS/U6

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-mMaroon-KIX-mMaroon

    Plasmid

    #137005

    Purpose

    mMaroon-KIX acceptor for R-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    KIX domain (Crebbp Rat)
    

    Tags

    mMaroon

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-hIFITM3-S61A,N64A,T65A

    Plasmid

    #104362

    Purpose

    Expresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated the following amino acids: S61A,N64A,T65A

    Promoter

    CMV

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDARMO_CMVT_3xFLAG-HUWE1_R4187C

    Plasmid

    #187142

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 R4187C

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R4187C

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGLS3-5xHRE-PHLDA3(wt)

    Plasmid

    #72557

    Purpose

    Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    PHLDA3 (PHLDA3 Human)
    

    Tags

    5XHRE

    Expression

    Mammalian

    Promoter

    E1b minimal

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONRG_P4-P1R:MUM4_0.3Pro_35S

    Plasmid

    #128558

    Purpose

    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning

    Depositor

    George Haughn

    Article

    Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.

    Type

    Empty backbone

    Use

    Gateway promoter entry clone

    Promoter

    MUM4 core promoter with 35S enhancer

    Available Since

    Sept. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMiR-E6AP-3'UTR

    Plasmid

    #53694

    Purpose

    Luciferase reporter assay for microRNA binding on E6AP 3'UTR

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    UBE3A 3'UTR (UBE3A Human)
    

    Use

    Luciferase

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. LentiCRISPRv2puro-Tbxa2r

    Plasmid

    #170304

    Purpose

    A knock-out vector for the mouse Tbxa2r

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxa2r gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-nirButterfly

    Plasmid

    #136591

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

    Depositor

    Vladislav Verkhusha

    Article

    Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

    Insert

    nirButterfly
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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