159,481 results

1. pAAV-EWB-DIO-cyan pre-eGRASP(p30)

   Plasmid

   #111583

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p30)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

   Plasmid

   #108542

   Purpose

   Lentivirus vector expressing Luciferase with blasticidin resistance

   Depositor

   Javier Alcudia

   Inserts

   Luciferase
   Neomycin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1apha and PGK

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Tet1

   Plasmid

   #60938

   Purpose

   Expresses Tet1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR58.6

   Plasmid

   #63176

   Purpose

   Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   CcaR
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1(+)-NEMOf

   Plasmid

   #189932

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 hygro

   Plasmid

   #24150

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

   Depositor

   Bob Weinberg

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCM157

   Plasmid

   #45863

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Insert

   cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-IRES-GFP II (pMIG II)

   Plasmid

   #52107

   Purpose

   a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRH030

   Plasmid

   #159633

   Purpose

   CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

   Depositor

   Robert Hertel

   Article

   Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

   Type

   Empty backbone

   Use

   CRISPR; Shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Constitutive tracrRNA

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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