164,577 results

1. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCENTR_aco_mScarlet3

   Plasmid

   #225984

   Purpose

   Arabidopsis codon-optimized mScarlet3 in an entry clone

   Depositor

   Imran Siddiqi

   Insert

   mScarlet3 Arabidopsis codon-optimized
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Arabidopsis Codon-Optimized mScarlet3

   Promoter

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET3a-OSF-CypA

   Plasmid

   #79039

   Purpose

   Bacterial vector for cloning and propagation in DH5alpha

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFG7m34GW

   Plasmid

   #133747

   Purpose

   Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

   Depositor

   Niko Geldner

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

   Plasmid

   #162379

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Promoter

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Puro_siKO-2TO

   Plasmid

   #86697

   Purpose

   Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 2TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHM830

   Plasmid

   #20702

   Depositor

   Thomas Stamminger

   Article

   Sorg et al Biotechniques. 1999 May . 26(5):858-62.

   Insert

   GFP-MCS-LacZ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-EGFP-CAAX

   Plasmid

   #208683

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT-WT-HA-GFP11

    Plasmid

    #177719

    Purpose

    ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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