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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18-LucFF

Plasmid

#220496

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

Firefly Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP NFATC2IP

Plasmid

#221433

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

1st Methionine of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3xFLAG-NFATC2IP

Plasmid

#221208

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

1st Methionine deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

UBC

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKelly-T2A-mtq2

Plasmid

#205506

Purpose

Expression of mKelly-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mKelly-T2A-mtq2

Expression

Yeast

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1419

Plasmid

#219778

Purpose

pDEF-TurboID-GFPmut2, negative control for cytosolic expression

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Promoter

PbEF1alpha promoter

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-LOVPLD-mCherry-OSBP(PH)

Plasmid

#219561

Purpose

Golgi-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

LOVPLD-mCherry-OSBP(PH)

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_A

Plasmid

#185803

Purpose

A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(ABI1)+-86MP+erGFP

Expression

Plant

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_R

Plasmid

#185804

Purpose

a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(RD29A)+-86MP+erGFP

Expression

Plant

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.3

Plasmid

#208304

Purpose

Expresses ABE-RA3.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.3-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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