We narrowed to 17,594 results for: cript

Showing: 7881 - 7900 of 17594 results

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA-BSD

Plasmid

#175583

Purpose

Expresses sgRNA for CasRx in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SnailHA_pcDNA3

Plasmid

#31697

Depositor

Paul Wade

Article

Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

Insert

Snail (SNAI1 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-mouseCENP-C

Plasmid

#174444

Purpose

in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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IVT-R2Tg_opt

Plasmid

#223247

Purpose

In vitro transcription of the optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Use

In vitro transcription

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p300 reporter

Plasmid

#11726

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

p300 promoter and 5' UTR (EP300 )

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGreen2x35SLUC

Plasmid

#108228

Purpose

Plant expression as translational fusions to FLUC with RLUC normalization

Depositor

Alejandro Ferrando

Article

Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

Type

Empty backbone

Use

Luciferase

Tags

Firefly Luciferase

Expression

Plant

Promoter

2x35S

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight

Plasmid

#205077

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V174C

Plasmid

#205078

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V174C

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV BAF170-Flag

Plasmid

#19142

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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