We narrowed to 26,307 results for: Vit

1. pYPQ269D

   Plasmid

   #164717

   Purpose

   Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
   

   Use

   CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-GSDMD_C192A-Myc

   Plasmid

   #201168

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   C192A

   Promoter

   MESV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT112_GalL_nCDA1-xBE3

   Plasmid

   #141318

   Purpose

   Expressing base editor nCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE:mTREK-1(K271Q)

   Plasmid

   #133270

   Purpose

   X. laevis expression vector. It will generate the mouse TREK-1 channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
   

   Use

   Oocyte expression

   Mutation

   K271Q

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 432 pSG5L HA RB 567L

   Plasmid

   #10725

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   RB 567L (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   567L

   Available Since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

6. AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46587

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-132 Decoy (MIR132, Mir132 Mouse, Human)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pspCas9-ABE-3'UTR-sgRNA-2xMS2

   Plasmid

   #132554

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
   

   Expression

   Mammalian

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Zebrafish PAC (fPAC)

   Plasmid

   #129498

   Purpose

   Expresses zebrafish TMEM206. In pcDNA3.1(+) backbone

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   zebrafish PAC (pacc1 Zebrafish)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. D5_Ld_280600

   Plasmid

   #135821

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   major surface protease gp63, putative,leishmanolysin, putative
   

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2puro-Ptger1

    Plasmid

    #170306

    Purpose

    A knock-out vector for the mouse Ptger1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptger1 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDARMO_CMVT_3xFLAG-HUWE1_H3962D

    Plasmid

    #187131

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 H3962D

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H3962D

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAWp63-clone32 (Opti-SpCas9)

    Plasmid

    #131736

    Purpose

    Expresses Opti-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    Opti-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    R661A+K1003H

    Promoter

    EFS

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-miR-148a

    Plasmid

    #65052

    Purpose

    Expression of miR148a in mammalian cells

    Depositor

    Cristina Fillat

    Article

    Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

    Insert

    hsa-miR-148a (pre-miRNA) (MIR148A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8e(TadA-8e K20A R21A)

    Plasmid

    #138496

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e K20A R21A)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e K20A R21A

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP250 pEF1a-TALE-SunTag

    Plasmid

    #100939

    Purpose

    Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-FRB:Akt1(D274A)

    Plasmid

    #86634

    Purpose

    Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FRB-Akt1(D274A) (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MDH1-shDusp5-2-H2Kb2

    Plasmid

    #17853

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shDusp5 (DUSP5 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-3xFLAG-Puro PAK5 K478R K469R

    Plasmid

    #167180

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R K469R
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1-3xMyc-ERK2(K52R)

    Plasmid

    #129228

    Purpose

    Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    ERK2(K52R) (Mapk1 Rat)
    

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Changed Lysine 52 to Arginine

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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