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TetO-FUW-Hes1

Plasmid

#61534

Purpose

Expresses Hes1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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LeGO-hEµMAR-iG2

Plasmid

#52635

Purpose

3rd generation lentiviral vector for enhanced transgene and eGFP-coexpression in primary B cells and B-cell lines

Depositor

Boris Fehse

Article

Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

Insert

hEµMAR-SFFV; IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Tags

Avi Tag and EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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psigma1-EGFP

Plasmid

#53611

Purpose

Expresses sigma1 of adaptor protein of AP1

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma 1 (Ap1s1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47455

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

AAV

Tags

2A_GFP

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pattB-hsp70P-QF#7-hsp70T

Plasmid

#46134

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Olig2

Plasmid

#45844

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-At-TRL

Plasmid

#32242

Depositor

Stan Fields, Jay Hesselberth

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pRc/CMV2 Dvl-2-Myc

Plasmid

#42194

Depositor

Robert Lefkowitz, Shin-ichi Yanagawa

Article

Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

Insert

Dvl 2 (Dvl2 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a

Plasmid

#40032

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-hAtg13

Plasmid

#38191

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMG10

Plasmid

#31238

Depositor

Keith Joung

Article

Soellu et al Nucleic Acids Res. 2010 Dec 1. 38(22):82

Type

Empty backbone

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 Wt

Plasmid

#29545

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-EPHB4

Plasmid

#23896

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB4 (EPHB4 Human)

Use

Gateway Cloning

Mutation

K162R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAG1

Plasmid

#23718

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG1 (PRKAG1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PFKM

Plasmid

#23728

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKM (PFKM Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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