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pDONR207 SARS-CoV-2 E 27nt-del

Plasmid

#153954

Purpose

Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114099

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

Plasmid

#71219

Purpose

dCas9 fused to the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Lentiviral

Tags

dSpCas9-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-EGFP

Plasmid

#66821

Purpose

dCas9 fused to BFP and EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-EGFP

Tags

dSpCas9-BFP-EGFP

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-FLAG-HA-CAD_WT-HA-Hygro

Plasmid

#253792

Purpose

pLenti lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD under a human PGK promoter.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Insert

CAD (CAD Human)

Use

Lentiviral

Tags

FLAG-HA and HA

Expression

Mammalian

Promoter

hPGK

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_dEGFP-deltaFluor-IRES-PuroR-WPRE

Plasmid

#251212

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-delta_fluor expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

EGFP-delta_fluor

Use

Lentiviral

Expression

Mammalian

Mutation

9bp deletion of T65 to G67

Promoter

EF-1a

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_RspCas13d-6xHIS

Plasmid

#251218

Purpose

Bacterial overexpression and recombinant protein purification of RspCas13d.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RspCas13d

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_BzCas13b-6xHIS

Plasmid

#251217

Purpose

Bacterial overexpression and recombinant protein purification of BzCas13b.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

BzCas13b

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_PspCas13b-6xHIS

Plasmid

#251216

Purpose

Bacterial overexpression and recombinant protein purification of PspCas13b.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

PspCas13b

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_LshCas13a-6xHIS

Plasmid

#251215

Purpose

Bacterial overexpression and recombinant protein purification of LshCas13a.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LshCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_LwaCas13a-6xHIS

Plasmid

#251214

Purpose

Bacterial overexpression and recombinant protein purification of LwaCas13a.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LwaCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_EGFP-IRES-PuroR-WPRE

Plasmid

#251211

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven EGFP expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE

Plasmid

#251210

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

dEGFP-Y66S

Use

Lentiviral

Expression

Mammalian

Mutation

Y66S

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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V12

Plasmid

#253336

Purpose

Fully genetically encoded protein tag for CryoET labelling

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

V12

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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Delta6

Plasmid

#253337

Purpose

Fully genetically encoded protein tag for CryoET labelling

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

Delta6

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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TOMO70_NTD_Delta6_GFP

Plasmid

#253341

Purpose

mitochondrial TOMO70 NTD tagged with Delta6 and GFP

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

TOMO70-NTD-Delta6-GFP (TOMM70 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

UBC

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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