We narrowed to 12,008 results for: SHA
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Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
TagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode
Plasmid#170254PurposeBarcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _34-78/myc-His
Plasmid#127496PurposeExpresses myc-tagged _34-78 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _34-78 (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 _34-78PromoterCMVAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 gp78 CD-m1,2 / JM25
Plasmid#13307DepositorInsertgp78 (AMFR Human)
TagsGSTExpressionBacterialMutationcDNA corresponds to aa309-643 derived from human …Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)
Plasmid#229742PurposeExpresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR1 mutant WEVL -> AEVAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)
Plasmid#229743PurposeExpresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR2 mutant WTFI -> ATFAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C 156-233
Plasmid#108257PurposeExpresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9DepositorInsertCHMP4C residues 156-233 (CHMP4C Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP4B-NS3-Blue
Plasmid#223534PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP4B (CHMP4B Human)
TagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP4B-mut-NS3-Green
Plasmid#223531PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP4B (CHMP4B Human)
TagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C, residues 156-233, no Cys
Plasmid#180642PurposeBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.DepositorInsertCHMP4C (CHMP4C Human)
TagsHIS-SUMOExpressionBacterialMutationresidues 316-366PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
TagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231AAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
TagsGB1-His6-TEVExpressionBacterialMutationS184CAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194894PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194886PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41-Adel-IDR
Plasmid#145228PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDRDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _34-66/myc-His
Plasmid#127495PurposeExpresses myc-tagged _34-66 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _34-66 (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 _34-66PromoterCMVAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 E57A/myc-His
Plasmid#127497PurposeExpresses myc-tagged NDUFS2/E57A in mammalian cellsDepositorInsertNDUFS2 E57A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 E57APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 T58A/myc-His
Plasmid#127498PurposeExpresses myc-tagged NDUFS2/T58A in mammalian cellsDepositorInsertNDUFS2 T58A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 T58APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 H60A/myc-His
Plasmid#127499PurposeExpresses myc-tagged NDUFS2/H60A in mammalian cellsDepositorInsertNDUFS2 H60A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 H60APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 W61A/myc-His
Plasmid#127500PurposeExpresses myc-tagged NDUFS2/W61A in mammalian cellsDepositorInsertNDUFS2 W61A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 W61APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 K62A/myc-His
Plasmid#127501PurposeExpresses myc-tagged NDUFS2/K62A in mammalian cellsDepositorInsertNDUFS2 K62A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 K62APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 P63A/myc-His
Plasmid#127502PurposeExpresses myc-tagged NDUFS2/P63A in mammalian cellsDepositorInsertNDUFS2 P63A (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 P63APromoterCMVAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKLR gRNA (BRDN0001148918)
Plasmid#77290Purpose3rd generation lentiviral gRNA plasmid targeting human PKLRDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKLR gRNA (BRDN0001148897)
Plasmid#77291Purpose3rd generation lentiviral gRNA plasmid targeting human PKLRDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKLR gRNA (BRDN0001149380)
Plasmid#77292Purpose3rd generation lentiviral gRNA plasmid targeting human PKLRDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKLR gRNA (BRDN0001149241)
Plasmid#77293Purpose3rd generation lentiviral gRNA plasmid targeting human PKLRDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-TGFb1
Plasmid#101762PurposeTGFb1 promoter reporterDepositorAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode
Plasmid#170226PurposeBarcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode
Plasmid#170237PurposeBarcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only