We narrowed to 8,596 results for: Set

1. LoxP2-Mb2Tomato-2A (SO240)

   Plasmid

   #99614

   Purpose

   To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2Tomato
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LoxP1-Mb2YFP-2A (SO244)

   Plasmid

   #99613

   Purpose

   To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2EYFP
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO_HA.NWS.Ollas_mCherry-NLS

   Plasmid

   #178263

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.NWS.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

   Plasmid

   #154777

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   102TAA 150TAG in GFP reporter, hybrid PylT with …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-HDC-DIO-GFP-shRNA.scramble

   Plasmid

   #184331

   Purpose

   Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and RNAi

   Expression

   Mammalian

   Promoter

   histidine decarboxylase promoter fragment that g…

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-PLXNB2-mGAP

   Plasmid

   #86241

   Purpose

   Lentivirus for expression of human Plexin-B2 with mutant GAP domain

   Depositor

   Roland Friedel

   Article

   Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

   Insert

   Plexin-B2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   PLXNB2 with point mutations of GAP domain

   Promoter

   hPGK

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO_NWS.HA.V5_mCherry-NLS

   Plasmid

   #178283

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.HA.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO_AU1.VSVg.V5_mCherry-NLS

   Plasmid

   #178236

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.VSVg.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO_AU1.S.VSVg_mCherry-NLS

   Plasmid

   #178234

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.S.VSVg and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid

    #196421

    Purpose

    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO_C.HA.V5_mCherry-NLS

    Plasmid

    #178242

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. qTAG-C-moxGFP-Puro-H3C2

    Plasmid

    #207783

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid

    #163766

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal GST tag
    

    Tags

    GST Tag

    Expression

    Insect

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-H1-shRNA-CMV-HA-PACSIN1

    Plasmid

    #72577

    Purpose

    Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

    Depositor

    Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Inserts

    PACSIN1 (Pacsin1 Mouse)
    H1-PACSIN1 shRNA cassette
    

    Use

    RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    5 silent mutations around shRNA recognition seque…

    Promoter

    CMV and H1

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid

    #178220

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_AU1.HA.S_mCherry-NLS

    Plasmid

    #178219

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_AU1.HA.HSV_mCherry-NLS

    Plasmid

    #178216

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.HSV and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_AU1.C.VSVg_mCherry-NLS

    Plasmid

    #178214

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_AU1.C.S_mCherry-NLS

    Plasmid

    #178213

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge