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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113342

Purpose

Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

Plasmid

#112921

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pT-Rex Dest30 dn-cHSF1

Plasmid

#134736

Purpose

Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV/TO

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.dn-cHSF1

Plasmid

#134726

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Expression

Mutation

Promoter

None

Available since

Availability

Academic Institutions and Nonprofits only

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pDest40 DHFR.dn-cHSF1

Plasmid

#134735

Purpose

Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro dn-cHSF1

Plasmid

#134728

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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