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Plasmid#199610PurposeEncodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.DepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationC3 cassette insertionPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
cTRE-GFP
Plasmid#135665PurposeIntroduce Dox-inducible (TRE promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertGFP
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-1
Plasmid#36409DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTagsmEGFPExpressionMutationPromoterno promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-13
Plasmid#36410DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTags3XmEGFPExpressionMutationPromoterNo promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-16
Plasmid#36411DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTags6X mEGFPExpressionMutationPromoterNo promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-GFP
Plasmid#99521PurposeC-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsGFPExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-EGFP
Plasmid#61462PurposeAAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpADepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-55:DMD-mEGFP
Plasmid#159744PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertDMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRA1SEGFPTcIfm
Plasmid#193777PurposeCassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPR promoter and PLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-HSP-GFP
Plasmid#65488Purposetransposable GFPDepositorInsertGFP transposable cassette (amaxaGFP)
UseTagsExpressionMutationPromoterhsp70Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_GFP 150TAG 6xHis
Plasmid#140015PurposePlasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTags6xHisExpressionMammalianMutation150TAGPromoterEF1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JL002: pMVP/eGFP-DEST
Plasmid#121852PurposepMVP destination vector, empty expression vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_GFP 150TAG
Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
LB601: pMVP/PB/eGFP-DEST
Plasmid#121870PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBac CMV-GFP-FRT
Plasmid#92078Purposeproduction of transgenic animal and recombination using flipaseDepositorInsertCMV-eGFP and TK-Neo between FRT3 and FRT
UsePiggybacTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_GFP 150TAG
Plasmid#140017PurposePlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
JT504: pMVP/SB/eGFP-DEST
Plasmid#121861PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTH
Plasmid#209319PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only