We narrowed to 214 results for: prion

1. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

   Plasmid

   #15602

   Depositor

   Susan Lindquist

   Article

   Tessier et al Nature. 2007 May 31. 447(7144):556-61.

   Insert

   Sup35 NM (SUP35 Budding Yeast, Candida albicans)
   

   Use

   Tags

   7xHis and Cysteine

   Expression

   Bacterial

   Mutation

   4 G/A. Four glycines mutated to alanines. The pos…

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJCNM(del22-68)

   Plasmid

   #1095

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acids 20 - 68 DELETED from NM domain of SUP…

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pJLI-Sup35N-KDG6-C

   Plasmid

   #1238

   Depositor

   Susan Lindquist

   Article

   Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Yeast integrative plasmid

   Tags

   Expression

   Mutation

   SUP35 middle region (aa124-253) was replaced with…

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pSK221

   Plasmid

   #129249

   Purpose

   reverse yTRAP, tetR-responsive Neon reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   pADH1(tetO2)-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAN147

   Plasmid

   #129202

   Purpose

   yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAN213

   Plasmid

   #129270

   Purpose

   Control for measuring background levels of yTRAP reporter protein

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   min pCYC1

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSK234

   Plasmid

   #131149

   Purpose

   dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSK260

   Plasmid

   #129252

   Purpose

   dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Sup35 NM yTRAP sensor reporting on mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSK247

   Plasmid

   #131148

   Purpose

   dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSUP35, min pCYC1

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p415TEF-NYFP

    Plasmid

    #99555

    Purpose

    Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

    Depositor

    Liming Li

    Article

    Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

    Insert

    Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS416 Gal-RNQ1 L94A-YFP

    Plasmid

    #18692

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 L94A (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Leucine 94 mutated to Alanine (L94A)

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pJC25R1+4stop

    Plasmid

    #17723

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    Sup35R1+4 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E.coli expression plasmid for NM-region of Sup35 …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAN201

    Plasmid

    #129204

    Purpose

    yTRAP sensor of [RNQ+], detects aggregation of Rnq1

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    RNQ1 (RNQ1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cytosolic moPrP (3F4)

    Plasmid

    #1321

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-term truncation (to make gene cytosolic), vario…

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSK226

    Plasmid

    #129268

    Purpose

    dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Rnq1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS306Sup35R1

    Plasmid

    #17715

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    pRS306Sup35R1 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    An pRS306 based integrating plasmid designed for …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWJPrP1

    Plasmid

    #22109

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    mutations at aa 108 and 111 to make 3F4 epitope (…

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pWJPrP5

    Plasmid

    #22106

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    mutated codon 177 (D to N)

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pX335_HR_Prnp_3

    Plasmid

    #78622

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA(MS2)_Prnp_SAM2

    Plasmid

    #78625

    Purpose

    Encodes sgRNA2.0 targeting 138nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sgRNA(MS2)_Prnp_SAM1

    Plasmid

    #78624

    Purpose

    Encodes sgRNA2.0 targeting 158nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYES2/103Q

    Plasmid

    #1385

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (103Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PRNP-bio-His

    Plasmid

    #51811

    Purpose

    Expresses full-length Major prion protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PRNP (PRNP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX459_HR_Prnp_3

    Plasmid

    #78623

    Purpose

    pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221-DDX5

    Plasmid

    #166163

    Purpose

    Gateway entry vector for N. furzeriDDX5

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221-mRuby3

    Plasmid

    #166136

    Purpose

    Gateway entry vector for mRuby3

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge