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PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-NES-ZapCV2 (cpV143)

Plasmid

#112060

Purpose

Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

Depositor

Amy Palmer

Article

Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

Insert

NES-ZapCV2

Use

Tags

Nuclear Export Signal (NES) derived from HIV-1

Expression

Mammalian

Mutation

ECFP gene is truncated 36 bp at 3' end. Venu…

Promoter

CMV

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-mTSG-GFAPCre

Plasmid

#100276

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Insert

GFAP promoter (Gfap, GFAP Human, Mouse)

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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XE68 APC-GFP in CS2+

Plasmid

#16687

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

APC-GFP (APC Human)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A-apCC-Di

Plasmid

#190706

Purpose

Encodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-apCC-Di

Use

Tags

Expression

Mutation

V150A

Promoter

araBAD

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

Plasmid

#105022

Purpose

Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Apc (Apc Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1941

Plasmid

#16510

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 1941 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-2644

Plasmid

#16511

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 2644 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1309

Plasmid

#16509

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 1309 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam APC 1-331

Plasmid

#16508

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 331 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBI121-Pro-Adh::TaPCS1:cmyc

Plasmid

#49765

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in plant expression vector

Depositor

Julian Schroeder

Article

Gong et al Proc Natl Acad Sci U S A. 2003 Aug 19;10

Insert

TaPCS1 (PCS1 Triticum aestivum)

Use

Plant expression

Tags

c-myc

Expression

Mutation

Promoter

Adh1

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TetO-Snu114-fSNAPc

Plasmid

#106989

Purpose

open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Snu114

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Gal4DBD-CapN-SsrA-CapC

Plasmid

#213533

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-B,C (1133–1189)

Plasmid

#132630

Purpose

expresses human APC-B,C (1133–1189) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-B,C (1133–1189)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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