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Showing: 61 - 80 of 217 results

  2. PPE18-ALFA

    Plasmid
    #231247
    Purpose
    Expresses a C-terminally tagged PPE18 (ALFA tag)
    Depositor
    Bryan Bryson
    Insert
    PPE18-ALFA (PPE18 Mycobacterium tuberculosis)
    Use
    Tags
    ALFA Tag
    Expression
    Bacterial
    Mutation
    Promoter
    hsp60
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231247-plasmid-map-sequence-id-463614

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  3. pAIP4_1LU4

    Plasmid
    #234207
    Purpose
    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1LU4 (mpt53 Mycobacterium tuberculosis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234207-plasmid-map-sequence-id-464172

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  4. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Article
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Tags
    Expression
    Mutation
    Promoter
    mycobacterial promoter Psmyc
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    70045-plasmid-map-sequence-id-124678

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  6. pSBY3_ligD

    Plasmid
    #104624
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104624-plasmid-map-sequence-id-201663

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  9. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Use
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  17. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

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  18. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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Showing: 61 - 80 of 217 results