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Cag.Luc.puro

Plasmid

#74409

Purpose

Constitutive Luciferase expression in mammalian cells along with puromycin selection

Depositor

Georgios Stathopoulos

Article

Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

Plasmid

#120564

Purpose

Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

Plasmid

#120563

Purpose

Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

Plasmid

#120565

Purpose

Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

Plasmid

#120567

Purpose

Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

Plasmid

#120566

Purpose

Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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JB95

Plasmid

#128922

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

nRasGD-BirA*-HA-MAO (NRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB96

Plasmid

#128923

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

nRasSN-BirA*-HA-MAO (NRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCGN N-Ras wt

Plasmid

#14723

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGN N-Ras 12D

Plasmid

#14725

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12D

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pCGN N-Ras 189L

Plasmid

#14724

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

189L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pCGN N-Ras 12D, 189L

Plasmid

#14726

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12D, 189L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pLB-915

Plasmid

#11620

Depositor

Stephan Kissler

Article

Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Insert

Slc11a1 shRNA (Slc11a1 Mouse)

Use

Cre/Lox and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC11A1

Plasmid

#131951

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC11A1 (SLC11A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC11A1_STOP

Plasmid

#161117

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC11A1 (SLC11A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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R777-E225 Hs.RAPGEF2

Plasmid

#70509

Purpose

Gateway ORF clone of human RAPGEF2 [NM_014247.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RAPGEF2 (RAPGEF2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E226 Hs.RAPGEF2-nostop

Plasmid

#70510

Purpose

Gateway ORF clone of human RAPGEF2 [NM_014247.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RAPGEF2 (RAPGEF2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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mV-MxCBD_655

Plasmid

#105823

Purpose

Expresses mVenus-fusion protein of MxCBD (Myxococcus xanthus CarH)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

mV-MxCBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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mV-TtCBD_656

Plasmid

#105824

Purpose

Expresses mVenus-fusion protein of MxCBD (Thermus thermophilus CarH)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

mV-TtCBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge