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Plasmid#60590PurposeA transcriptional AND circuit constructed by layering NOR and NOT gatesDepositorInsertAND_Int2
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAND_yfp
Plasmid#60592Purposeused with pSpec to generate an AND gate without memoryDepositorInsertAND_yfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN1579
Plasmid#127201PurposeProgesterone inducible expression of KeyC-BFPDepositorInsertpPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAN1735
Plasmid#127204PurposeDual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN1779
Plasmid#127165PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)DepositorInsertpGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY3
Plasmid#112197PurposeThis plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN1935
Plasmid#127203PurposeConstitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchCDepositorInsertpTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN1572
Plasmid#127202PurposeDual inducible expression of Venus-degronSwitchA and KeyA-BFPDepositorInsertpGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANY2
Plasmid#112196PurposeThis plasmid is used for efficient cloning of DNA and expression of protein in E. coli.DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY1
Plasmid#110949PurposeUniversal mini-vector for efficient cloning of DNA and expression of protein in E. coli.DepositorInsertccd B cassette
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAN008
Plasmid#220049PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).DepositorInsertflag-HA-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN007
Plasmid#220048PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.DepositorInsertflag-HA-stop-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN013
Plasmid#220050PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.DepositorInsertGFP-stop-fLuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN056
Plasmid#220052PurposeReporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).DepositorInsertfLuc-CFTR (exons 22-27) (CFTR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN057
Plasmid#220053PurposeNMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).DepositorInsertfLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
UseTagsExpressionMammalianMutationc.3846G>A mutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN012
Plasmid#220051PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).DepositorInsertGFP-fLuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN053
Plasmid#220047PurposeMammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcriptDepositorInsertcrRNA-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only