Addgene

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pIS1-wt RhoA 3'UTR

Plasmid

#26089

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

wt RhoA 3'UTR (RHOA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
CaM-RhoA(DP)-Venus

Plasmid

#31994

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

Insert

CaM-RhoA(DP) (RHOA Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L mutation in RhoA, E7S and D65Y in CaM

Promoter

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
mRFP-PA-GFP-RhoAQ63L

Plasmid

#187282

Purpose

Express pmRFP-PAGFP-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

RhoA (RHOA Human)

Use

Tags

mRFP-PAGFP

Expression

Mammalian

Mutation

RhoA Q63L

Promoter

CMV, T7

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GFP11-RhoA

Plasmid

#181860

Purpose

Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-RhoA (RHOA Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa-DN

Plasmid

#109526

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

DN

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAb

Plasmid

#109527

Purpose

Muscle specific zebrafish expression of mKate2 fused to RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-RHOA-Y42S

Plasmid

#116674

Purpose

Lentiviral expression of RHOA Y42S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RHOA (RHOA Human)

Use

Lentiviral

Tags

Expression

Mutation

Y42S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-Q63L

Plasmid

#12968

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA constitutively active (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-T19N

Plasmid

#12967

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA DN (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T19N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-RhoA-Q63L

Plasmid

#12964

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q63L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-RhoA-T19N

Plasmid

#12963

Depositor

Gary Bokoch

Insert

RhoA DN (RHOA Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T19N Dominant negative

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Use

Tags

GFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Tet3G

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T3-RhoA-Q63L

Plasmid

#12961

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Q63L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-RhoA G17A

Plasmid

#69357

Purpose

expression of nucleotide free RhoA in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

RhoA (RHOA Human)

Use

Tags

GST

Expression

Bacterial

Mutation

G17A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pB-Tet-On-HTR6-RhoA sensor

Plasmid

#189614

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6 (HTR6 Human)

Use

Tags

GFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Tet3G

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet-Off promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI RhoA

Plasmid

#189669

Purpose

Expression of Antenna-RhoA

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

RhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge