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Plasmid#148848PurposeMammalian Expression of HsAGO2-sgRNA1DepositorInsertHsAGO2-sgRNA1 (AGO2 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH
Plasmid#148851PurposeMammalian Expression of HsAGO2-sgRNA2DepositorInsertHsAGO2-sgRNA2 (AGO2 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH
Plasmid#148854PurposeMammalian Expression of HsAGO1-sgRNADepositorInsertHsAGO1-sgRNA (AGO1 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1-P588GA618FK658AL692Y_AH
Plasmid#148860PurposeMammalian Expression of HsAGO1-P588GA618FK658AL692YDepositorInsertHsAGO1-P588GA618FK658AL692Y (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO3-P591GA621FK661AL695Y_AH
Plasmid#148861PurposeMammalian Expression of HsAGO3-P591GA621FK661AL695YDepositorInsertHsAGO3-P591GA621FK661AL695Y (AGO3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO4-P582GG612FK662AL696Y_AH
Plasmid#148862PurposeMammalian Expression of HsAGO4-P582GG612FK662AL696YDepositorInsertHsAGO4-P582GG612FK662AL696Y (AGO4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
Plasmid#146286PurposeInsect Expression of HsAGO2-F2V2DepositorInsertHsAGO2-F2V2 (AGO2 Human)
UseTagsExpressionInsectMutationContains F470V, F505V, K660E mutations compared t…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR I206F (JBEI-3678)
Plasmid#110149PurposeSaGGR site-directed mutation of I206FDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of I206FPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR G91H (JBEI-3677)
Plasmid#110148PurposeSaGGR site-directed mutation of G91HDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of G91HPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR L377H (JBEI-3683)
Plasmid#110150PurposeSaGGR site-directed mutation of L377HDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of L377HPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CJ26 (miR-1003 3'ssAG->GA)
Plasmid#17765DepositorInsertdme-miR-1003 minigene 3'ssAG->GA
UseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationsplice site mutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)
Plasmid#231379PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56A, R68A, R97A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-HsAGO2-448-859-optim_K
Plasmid#146729PurposeBacterial Expression of HsAGO2_448-859-optimDepositorInsertHsAGO2_448-859-optim (AGO2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TAPtagN-PrP-SBP-HsAGO2_AC
Plasmid#148440PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationone silent mutation C840Tand sequence differs in …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-I206F-L377H (JBEI-7202)
Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ROC2
Plasmid#19899DepositorInsertRING finger protein 2 (ROC2) (RNF7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
BtARR1
Plasmid#236262PurposeBacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATGDepositorInsertarrestin-1 (SAG Bovine)
UseTagsExpressionBacterialMutationinserted GGT codon after starting ATGPromoterlacAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only