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pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Plasmid

#198417

Purpose

Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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JE301: pMVP (L5-L4) Tir1-P2A-AID

Plasmid

#121722

Purpose

pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1-P2A-AID (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc (C-term on osTIR1)

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA

Plasmid

#222708

Purpose

Expression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtTIR1 (TIR1 Mustard Weed)
AtIAA6 (IAA6 Mustard Weed)

Use

Tags

HA, MBP, and myc

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUbC-OsTIR1-myc-IRES-scFv-sfGFP

Plasmid

#84563

Purpose

Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

Depositor

Robert Singer

Article

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP

Use

Lentiviral

Tags

myc tag (9x)

Expression

Mutation

Promoter

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

Plasmid

#140533

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

Plasmid

#169352

Purpose

Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

Depositor

Tony Carr

Insert

OsTIR1(WT)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

Plasmid

#216247

Purpose

Express OsTIR1.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

OsTIR1.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

Plasmid

#129667

Purpose

A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

Plasmid

#169353

Purpose

Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

Depositor

Tony Carr

Insert

OsTIR1(F74A)

Use

Tags

Expression

Yeast

Mutation

F74A

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR

Plasmid

#160042

Purpose

For the auxin receiver that report on auxin.

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
BlastR

Use

Luciferase

Tags

mAID and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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