We narrowed to 296 results for: actb

1. pX330_ACTB-i3 sgRNA / hSpCas9

   Plasmid

   #172827

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_ACTB-i2 sgRNA / hSpCas9

   Plasmid

   #172826

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-f1(+)-ACTB-iTagRFP-T

   Plasmid

   #115915

   Purpose

   donor to insert iTagRFP-T at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-TagRFP-T (ACTB Human)
   

   Use

   Donor template

   Tags

   TagRFP-T

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKI-cjACTB-EGFP

   Plasmid

   #129747

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Solo-mStayGold-ACTB

   Plasmid

   #227326

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDY0663 ACTB atgRNA with v2 scaffold

   Plasmid

   #179108

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Blast-mScarlet-ACTB

    Plasmid

    #207753

    Purpose

    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. qTAG-N-Blast-mNeon-ACTB

    Plasmid

    #207751

    Purpose

    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-DIO-LACTB-dAPEX2

    Plasmid

    #117180

    Purpose

    Plasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Puro-mNeon-ACTB

    Plasmid

    #207752

    Purpose

    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-ACTB-C200-GFPfr1

    Plasmid

    #169798

    Purpose

    THe donor vector for the ACTB gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to ACTB gene for HR
    

    Use

    Hr donor vector for human actb gene.

    Mutation

    Partial sequence of GFP is inserted before the st…

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZc-HsACTB*-thymosinB-8His

    Plasmid

    #111146

    Purpose

    Expresses human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PBS Actb-2A-TIR1-GFP

    Plasmid

    #113832

    Purpose

    targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    osTIR1-GFP (NEWENTRY Synthetic)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIS1-Vim/Actb5UTR-renilla

    Plasmid

    #38238

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Actb promoter (1000 nt upstream) and 5' UTR …

    Promoter

    Endogenous Vim

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only