We narrowed to 1,136 results for: cas12

1. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-AsCas12f-sgRNA-v2

   Plasmid

   #204639

   Purpose

   Expression of trancated AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   trancated AsCas12f sgRNA (sgRNA-v2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas12j-8-crRNA

   Plasmid

   #194967

   Purpose

   The plasmid expresses Cas12j-8-crRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPomyces-AsCas12j-2

   Plasmid

   #191655

   Purpose

   All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

   Depositor

   Fong Tian Wong

   Article

   Tan et al bioRxiv 2021.10.28.465406

   Insert

   AsCas12j-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas12a(RuvC)

   Plasmid

   #186447

   Purpose

   Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D917A, E1006A

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas-Cas12m

   Plasmid

   #192274

   Purpose

   Encodes MmCas12m under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC_dfnCas12a-KRAB_crRNA NC

   Plasmid

   #176262

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS nErCas12an

   Plasmid

   #132642

   Purpose

   Used for in vitro transcription of dual NLS ErCas12a mRNA

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   CRISPR; In vitro transcription

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNHEJ-Cas12a

   Plasmid

   #158713

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas12b(RuvC)

    Plasmid

    #186450

    Purpose

    Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E848A, D977A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LbCas12a-nuCPE

    Plasmid

    #213748

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-LbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R in LbCas12a

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. rnLbCas12a-PE2

    Plasmid

    #213746

    Purpose

    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    rnLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, H759A, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. His_CL7_2NLS_iCas12a-4NLS

    Plasmid

    #222971

    Purpose

    Bacterial expression of NLS-rich iCas12a construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His_CL7_2NLS_iCas12a-4NLS
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. act5c-LbCas12a

    Plasmid

    #140620

    Purpose

    Ubiquitous expression of LbCas12a in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LbCas12a-D156R

    Plasmid

    #213744

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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