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We narrowed to 6,447 results for: nls

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Showing: 61 - 80 of 6447 results

  1. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  2. pDAC627

    Plasmid
    #195240
    Purpose
    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195240-plasmid-map-sequence-id-457484

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  4. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

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  9. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  13. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid
    #47457
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47457-plasmid-map-sequence-id-69629

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  14. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  15. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

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  20. pdCas9-humanized

    Plasmid
    #44246
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dead Cas9 with 3X NLS
    Use
    CRISPR and Retroviral; Vector
    Tags
    3x NLS
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44246-plasmid-map-sequence-id-60247

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Showing: 61 - 80 of 6447 results