We narrowed to 6,070 results for: nls

1. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

   Plasmid

   #132406

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-nls-st1dCas9-3nls-3XGFP

   Plasmid

   #64111

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-3xFLAG-NLS-SpCas9-NLS

   Plasmid

   #51307

   Purpose

   Cas9 expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Insert

   3xFLAG-NLS-SpCas9-NLS
   

   Use

   CRISPR; Xenopus tropicalis expression

   Tags

   3x Flag and NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-dCas9-HA-NLS-GFP-VP16

   Plasmid

   #183923

   Purpose

   VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-VP16
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

   Plasmid

   #87109

   Purpose

   BPNLS-Cas9-BPNLS-2AGFP expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS-2A-EGFP
   

   Use

   CRISPR

   Tags

   3xFLAG (on Cas9)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

   Plasmid

   #196829

   Purpose

   Express dpdpCas13b with AAAA mutation and Clover

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Use

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

   Plasmid

   #176458

   Purpose

   Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSDest2-NmeCas9-NLS-3XHA-NLS

   Plasmid

   #87448

   Purpose

   Mammalian expression of wild type Nme Cas9

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Nme Cas9
   

   Use

   CRISPR

   Tags

   3XHA, SV40 NLS, and cMyc-like NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid

    #183924

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP4-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mesp-1916/-1>nls::Cas9::nls

    Plasmid

    #59988

    Purpose

    Mesp driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Mesp -1916/-1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid

    #132927

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Insert

    humanized SpCas9
    

    Use

    CRISPR and Synthetic Biology; Pmagic gateway entr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

    Plasmid

    #121827

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-Cas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239024

    Purpose

    Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin2-SEC61B (SEC61B Human, Synthetic)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p23-NLS-RfxCas13d-mcherry-NLS-Flag

    Plasmid

    #165077

    Purpose

    overexpression of RfxCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RfxCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

    Plasmid

    #171586

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS-HA

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    His-MBP-NLS-RfxCas13d-NLS-HA
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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