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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1

Plasmid

#60540

Purpose

Donor vector for human MLK1

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

E179K

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 MLK1 D294A

Plasmid

#60542

Purpose

Donor vector for human MLK1 D294A mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

D294A

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6p1_RBFOX2_RRM

Plasmid

#224293

Purpose

GST-RBFOX2(RRM domain) purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 RNA-recognition motif (RBFOX2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 74-167

Promoter

tac promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

Plasmid

#128386

Purpose

Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

Depositor

Craig Duvall

Article

Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

Inserts

NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

C-terminal luc2 fragment and N-terminal luc2 frag…

Expression

Mammalian

Mutation

N terminal carbohydrate recognition domain fused …

Promoter

EF1A and IRES

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pOpen-NotIR (GC)

Plasmid

#165507

Purpose

Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

NotI (GC-rich recognition site)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMT643

Plasmid

#8610

Depositor

Robert Hartley

Article

Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

Insert

barstar A

Use

Tags

Expression

Bacterial

Mutation

Two cysteines of barstar changed to alanine, C40A…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pRSET Nucleolin

Plasmid

#13037

Depositor

Peter Newburger

Article

Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Insert

Nucleolin (NCL Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-PL

Plasmid

#20783

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pDPlk-lin-41_LCS-wt

Plasmid

#26564

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDPlk-lin-41_LCS-sm

Plasmid

#26565

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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p67.uni.pA.OTIIp.mHEL.flag.mCMV.PGK.CD63GFP.WPRE

Plasmid

#228765

Purpose

mHEL and OT2-peptide

Depositor

Ferdinando Pucci

Insert

OVA epitope ISQAVHAAHAEINEAGR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_wt

Plasmid

#26558

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7

Use

Transcription vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCX138

Plasmid

#229213

Purpose

CRISPR array plasmid for NOTCH2 (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for NOTCH2 (Spacers 25-48)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCrl

Plasmid

#122443

Purpose

Expresses entire mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Article

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Article

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Use

Tags

Expression

Bacterial

Mutation

None, WT gene

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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mscv2.2

Plasmid

#60206

Purpose

Mammalian cell expression plasmid; drives constitutive, low-level expression from retroviral LTR; retroviral vector

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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TLR4

Plasmid

#20863

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Mar 1. ():.

Insert

Toll-Like Receptor 4 (TLR4 Human)

Use

Tags

Expression

Insect

Mutation

Contains ectodomain (amino acids 27–631)

Promoter

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_LPB

Plasmid

#26560

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPB

Use

Transcription vector

Tags

Expression

Mutation

loop mutant B

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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