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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)

Plasmid

#231377

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, K98A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)

Plasmid

#231378

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97A, K98A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)

Plasmid

#231380

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97E, K98E

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)

Plasmid

#231383

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A, H712A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2EF-DY

Plasmid

#221723

Purpose

Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2EF-DY (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D and F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO4_F

Plasmid

#146317

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2_E

Plasmid

#146224

Purpose

Insect Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2 (AGO2 Human)

Expression

Insect

Mutation

Contains a K660E mutation compared to the sequenc…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO1_C

Plasmid

#146083

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO4_C

Plasmid

#146085

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO3_C

Plasmid

#146084

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Expression

Mammalian

Mutation

T25A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

Plasmid

#148855

Purpose

Mammalian Expression of HsAGO3-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO3-sgRNA (AGO3 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CJ34 (miR-1003 3'ssAG->GA)

Plasmid

#17770

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila p-element vector

Mutation

splice site mutation

Available Since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+-FLAG3-drN-hsAgo2

Plasmid

#221724

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drN-hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for zebrafish Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO2-F2V2_L

Plasmid

#146885

Purpose

Mammalian Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsAGO2-F2V2 (AGO2 Human)

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-F2V2_F

Plasmid

#146287

Purpose

Mammalian Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F2V2 (AGO2 Human)

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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