159,258 results

1. pGHE-PdNIP2-1

   Plasmid

   #108447

   Purpose

   PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

   Depositor

   Richard Bélanger

   Insert

   PdNIP2-1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159845

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PHB1

   Plasmid

   #212153

   Purpose

   For PHB production in E. coli (PT7-pMB1).

   Depositor

   Kang Zhou

   Insert

   poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-RIα-ICUE4

   Plasmid

   #181848

   Purpose

   ICUE4 cAMP sensor tethered to the PKA RIα subunit.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
   

   Use

   Tags

   ECFP, PKA RIα, and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3349 pSFFV-neo Bcl-2

   Plasmid

   #8769

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. R777-E235 Hs.RASAL2

   Plasmid

   #70519

   Purpose

   Gateway ORF clone of human RASAL2 [NM_170692.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASAL2 (RASAL2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Use

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDuet TDP-43 G294A

   Plasmid

   #27463

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Tags

   Expression

   Bacterial

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

10. R777-E237 Hs.RASAL3

    Plasmid

    #70521

    Purpose

    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL3 (RASAL3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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