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pLL3.7-EF-EYFP-YAP1_S94A-PolyA

Plasmid

#112286

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Serine 94 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

Plasmid

#58959

Purpose

lentiviral expression of v-H-Ras and luciferase/EGFP reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene

Use

Lentiviral

Expression

Mammalian

Promoter

CMV enhancer/chicken beta actin

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-2

Plasmid

#210134

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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PH-Btk-mRuby2

Plasmid

#124657

Purpose

PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

BTK (BTK Human)

Tags

mRuby2

Expression

Mammalian

Mutation

contains only the PH domain of Bruton tyrosine ki…

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

Plasmid

#193719

Purpose

Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)

Tags

2xHA-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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aL-EGFP

Plasmid

#103879

Purpose

full-length human aL with EGFP at betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with EGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Article

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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aLL-EGFP

Plasmid

#103880

Purpose

aL-EGFP with 4aa linker

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with EGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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aL-L3

Plasmid

#103881

Purpose

full-length human aL with moxsynGFP in betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with moxsynGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

Plasmid

#196518

Purpose

Expression of Ctdnep1_D67E-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67EsiR (CTDNEP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead). Silent mutatio…

Promoter

CMV promoter; T7 promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Moe-pDONR221

Plasmid

#124239

Purpose

Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Insert

Drosophila Moesin (Moe Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

Plasmid

#112287

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Tyrosine 357 to Phenyalanine corresponding to tyr…

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Lats1-guides

Plasmid

#254766

Purpose

Lat1 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Lats1 (lats1 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Gcat1-guides

Plasmid

#254767

Purpose

Gcat1 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Gcat1 (gcat Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_M-Open-DDD

Plasmid

#234561

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-DDD (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

D392A, D500A, D503A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_M-Open-KRR

Plasmid

#234562

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-KRR (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

K525A, R548A, R551A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Eps8L2_1-496

Plasmid

#196516

Purpose

Expression of GST-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Mutation

Silent mutations for siRNA resistance

Promoter

lac promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2_1-496-siR

Plasmid

#196515

Purpose

Expression of Myc-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2-FL-siR

Plasmid

#196514

Purpose

Expression of Myc-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2-FL-siR (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.A18T

Plasmid

#99360

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.E31K

Plasmid

#99361

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 31 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.110

Plasmid

#99363

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine and Glutamic acid 31 to L…

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Lifeact-7

Plasmid

#54491

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

mCherry-Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-Lifeact-7

Plasmid

#54610

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Lifeact-mEGFP

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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