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Dead Streptavidin-SpyCatcher
Plasmid#59547PurposepET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramersDepositorInsertDead-dloop-Streptavidin-SpyCatcher
UseTagsN-terminally shortened SpyCatcherExpressionBacterialMutationPolyAsp in L3/4 loop for ion-exchangePromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc LbaCas12a
Plasmid#182123PurposepET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsUseTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc AspCas12a
Plasmid#182122PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a
Plasmid#182126PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
Plasmid#182121PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
Plasmid#182124PurposepET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteriaDepositorInsert1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsUseTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCRW1
Plasmid#122462PurposeHuman NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cellsDepositorUseTagsHis TagExpressionBacterialMutationPromoterT7lac promoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Top7 H1-H2
Plasmid#12479DepositorInsertTop7 H1-H2
UseTags6xHisExpressionBacterialMutationH1-H2PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Top7 S2-H2
Plasmid#12477DepositorInsertTop7 S2-H2
UseTags6xHisExpressionBacterialMutationS2-H2PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Top7 S2-S4
Plasmid#12478DepositorInsertTop7 S2-S4
UseTags6xHisExpressionBacterialMutationS2-S4PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Top7 H1-S4
Plasmid#12480DepositorInsertTop7 H1-S4
UseTags6xHisExpressionBacterialMutationH1-S4PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
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FDA60
Plasmid#45606DepositorInsertFDA60
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
FR52re
Plasmid#45489DepositorInsertFR52re
UseTags6xHisExpressionBacterialMutationredesign of Di-I_5_FR28PromoterAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
ssCFr
Plasmid#12481DepositorInsertssCFr
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
CFr
Plasmid#12482DepositorInsertCFr
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
TOP8
Plasmid#12485DepositorInsertTop8
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
VJQ_Nterm
Plasmid#12483DepositorInsertVJQ Nterm
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
PL2x2_5
Plasmid#45491DepositorInsertPL2x2_5
UseTags6xHisExpressionBacterialMutationchanged E30Y of Di-IV_5_PL2x2_6PromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
FR55
Plasmid#45490DepositorInsertFR55
UseTags6xHisExpressionBacterialMutationchanged F14L and added Gly at N-terminus of Di-I_…PromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Top7 Eb13 triple
Plasmid#12475DepositorInsertTop7 Eb13 triple
UseTags6xHisExpressionBacterialMutationEb13 triple. Top7 extended.PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Top7 Eb8_2 triple
Plasmid#12476DepositorInsertTop7 Eb8_2 triple
UseTags6xHisExpressionBacterialMutationEb8_2 triple. Top7 extended.PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
JGI_018129
Plasmid#158934PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_108826550_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018131
Plasmid#158935PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_119951226_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018133
Plasmid#158936PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_101620581_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018135
Plasmid#158937PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_096909276_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018137
Plasmid#158938PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_126985334_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018139
Plasmid#158939PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_058441655_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018141
Plasmid#158940PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertABL00697_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018143
Plasmid#158941PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_061782733_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018145
Plasmid#158942PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_003805730_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018147
Plasmid#158943PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertOGN97459_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018149
Plasmid#158944PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_005508112_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018151
Plasmid#158945PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertCVN04163_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018153
Plasmid#158946PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_005885358_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018089
Plasmid#158914PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_006592558_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018093
Plasmid#158916PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_091632192_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018095
Plasmid#158917PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_015749354_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018099
Plasmid#158919PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_012868615_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only
