We narrowed to 4,326 results for: CIT

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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2

Plasmid

#51309

Purpose

Expresses far-red fluorescent protein mNeptune2 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2301

Plasmid

#65778

Purpose

Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

St1Cas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 deltaKH

Plasmid

#17688

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

aa 157-256 deleted (KH domain)

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pRS416 Gal Q331K YFP

Plasmid

#27450

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
Rasgrf2-2A-dCre targeting vector

Plasmid

#61573

Purpose

Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9

Plasmid

#140237

Purpose

Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF-1a core

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pRG2

Plasmid

#104174

Purpose

sgRNA expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-As-crRNA

Plasmid

#78956

Purpose

Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-BE4

Plasmid

#108386

Purpose

Mammalian expression vector for xCas9(3.6)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE4

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE2

Plasmid

#197146

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ChrimsonR-tdTomato

Plasmid

#99231

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CaMKII

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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