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pCDNA4.TO-K9-2xCSTREP

Plasmid

#136160

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA2

Plasmid

#22508

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA2 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pB80-iLID-mCherry-RAB11

Plasmid

#174623

Purpose

Optogenetic coupling to recycling endosomes (RAB11) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K7-2xCSTREP

Plasmid

#136156

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K7

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K7

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K6-2xCSTREP

Plasmid

#136155

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K6

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K6

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K4-2xCSTREP

Plasmid

#136151

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K4.1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Plasmid

#174642

Purpose

Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PTet-F30-Pepper aptamer-UTR1-mTurq2

Plasmid

#227645

Purpose

pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

Depositor

Richard Murray

Article

Kapasiawala et al ACS Synth Biol. 2024 Dec 20;13(12):3976-

Insert

F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-542 POLK

Plasmid

#201585

Purpose

Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-542 of DNA polymerase kap…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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deltaLoop-MIDAS

Plasmid

#171605

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Replaced amino acids 4458-4496 with ASGSGS linker

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-LaminA

Plasmid

#231894

Purpose

Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

LaminA (LMNA Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)-GCN4-L-mCherry

Plasmid

#193715

Purpose

Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

hsKIF5B(aa1-353); mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF9-2xCSTREP

Plasmid

#136173

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF9

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4.TO-ORF57-2xCSTREP

Plasmid

#136217

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF57

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge