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pXPR_003 sgPIGA guide 1

Plasmid

#193610

Purpose

PIGA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 1 (PIGA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#1

Plasmid

#171503

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#1

Plasmid

#171505

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DeltaNIRD C95S KVS-1

Plasmid

#113068

Purpose

Expresses worm truncated KVS1.2 C113S in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

Insert

C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)

Use

Tags

Expression

Mammalian

Mutation

C113S+first 40 AA deletion

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Synthetic, Human)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Use

Tags

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-DmrC

Plasmid

#61673

Purpose

Non-fluorescent, truncated kinesin-1 fused to DmrC linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-DmrC (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1899)

Plasmid

#170143

Purpose

Expresses residues 1-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1900-2997

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1547)

Plasmid

#170144

Purpose

Expresses residues 1-1547 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1548-2997

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-mCitrine

Plasmid

#61676

Purpose

Single mCitrine-labeled, truncated kinesin-1

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KHC(1-560)-mCitrine (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-GFP11

Plasmid

#61670

Purpose

Non-fluorescent, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-GFP11 (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)

Plasmid

#99513

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfSec15A_5

Plasmid

#26716

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

Dog Sec15A RNAi (EXOC6 C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Sec15A-specific RNAi.

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only