We narrowed to 2,181 results for: levi

1. SLK_HUMAN_D0

   Plasmid

   #79733

   Purpose

   This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SLK (SLK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSc-His6-Sem1Sc

   Plasmid

   #83235

   Purpose

   pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Sem 1 (SEM1 Budding Yeast)
   

   Use

   Tags

   His6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STK16_HUMAN_D0

   Plasmid

   #79701

   Purpose

   This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK16 (STK16 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pRS406-PHO5-GFP-CERT PH domain EE mutation

   Plasmid

   #58833

   Purpose

   Expresses GFP-Tagged and mutated CERT PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   changed Arginine 43 to Glutamate

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. KCC4_HUMAN_D0

   Plasmid

   #79744

   Purpose

   This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC4 (SLC12A7 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. KS6A1_HUMAN_D1

   Plasmid

   #79720

   Purpose

   This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KS6A1 (RPS6KA1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. STK10_HUMAN_D0

   Plasmid

   #79722

   Purpose

   This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK10 (STK10 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLenti PGK FKBP12(F36V)-OGT (Neo)

   Plasmid

   #154294

   Purpose

   Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid

    #154285

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations (HCF-…

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid

    #154280

    Purpose

    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; bact…

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CSK_HUMAN_D0

    Plasmid

    #79683

    Purpose

    This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK (CSK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. KC1G3_HUMAN_D0

    Plasmid

    #79685

    Purpose

    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G3 (CSNK1G3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLenti PGK mKate2-OGT K842M (Puro)

    Plasmid

    #154291

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    K842M mutation (Catalytic Inactive)

    Promoter

    PGK

    Available since

    Dec. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. NEK1_HUMAN_D0

    Plasmid

    #79725

    Purpose

    This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid

    #154292

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    D554N mutation (Ser/Thr glycosylation-incompetent)

    Promoter

    PGK

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MERTK_HUMAN_D0

    Plasmid

    #79705

    Purpose

    This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MERTK (MERTK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only