We narrowed to 18,336 results for: met

1. Ppst93-ANME1G37 MCR-NG

   Plasmid

   #192773

   Purpose

   Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pVC

   Plasmid

   #175063

   Purpose

   expresses mVenus and mCherry pair in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   mVenus + mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. htpG2-GFP

   Plasmid

   #109388

   Purpose

   GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   phtpG2-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

   Plasmid

   #174526

   Purpose

   Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Insert

   PylS
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-104

   Plasmid

   #79842

   Purpose

   Expresses NLS-PpsR2-VP16 under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16
   

   Use

   Tags

   Nuclear localization signal of nuclear cap-bindin…

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV MN (eGFP)

   Plasmid

   #89932

   Purpose

   Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   MN
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   M.SssI residues 2-272 fragment

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaYin1 NES

   Plasmid

   #33318

   Depositor

   Robert Campbell

   Article

   Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

   Insert

   CaYin1 NES
   

   Use

   Tags

   NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

   Plasmid

   #174529

   Purpose

   Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Inserts

   PylS
   PylT
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ppst93-E50 MCR-NG

   Plasmid

   #192778

   Purpose

   Expresses NG-tagged E50 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Ppst93-ANME2cLM014 MCR-NG

    Plasmid

    #192777

    Purpose

    Expresses NG-tagged ANME2cLM014 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Ppst93-ANME2cE20 MCR-NG

    Plasmid

    #192775

    Purpose

    Expresses NG-tagged ANME2cE20 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Ppst93-ANME1BS MCR-NG

    Plasmid

    #192772

    Purpose

    Expresses NG-tagged ANME1BS MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174528

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p415 Glo3 R59K S389,398 D

    Plasmid

    #112657

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K S389,398D
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (S389D, S398D) in Glo3 motif, pho…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p415 GFP-Glo3 S389,398 D

    Plasmid

    #112661

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 S389,398D
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389D, S398D) in Glo3 motif, phosphomi…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PHBA03

    Plasmid

    #83537

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GA-NES

    Plasmid

    #61018

    Purpose

    ddGFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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