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pCAG-PLE-IRES-mCherry

Plasmid

#45766

Depositor

Luke Lavis

Publication

Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

Insert

esterase

Expression

Mammalian

Promoter

CAG

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

Plasmid

#65418

Purpose

AAV packaging vector for CamKIIa driven KOR DREADD expression

Depositor

Bryan Roth

Publication

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Insert

CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Promoter

CaMKIIa

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SV-ABAactDA

Plasmid

#38247

Depositor

Jerry Crabtree

Publication

Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

Inserts

VP16/PYL1
Gal4DBD/ABI*

Tags

Flag, Gal4DBD, HA, and VP16

Expression

Mammalian

Mutation

D143A

Promoter

SV40 (IRES) and SV40p

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Publication

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-Trastuzumab-IgG4/κ

Plasmid

#61887

Purpose

Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p2attPC

Plasmid

#51547

Purpose

DICE donor plasmid without neural transcription factors.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

mCherry

Expression

Mammalian

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJYS1Ptac

Plasmid

#85545

Purpose

Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

tac and unknown

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Publication

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Expression

Bacterial

Mutation

K1750A

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Publication

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Publication

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-H2BtdiRFP-IP

Plasmid

#47884

Purpose

Histone H2B fused with tandem iRFP, ires-puro

Depositor

Maria-Elena Torres-Padilla

Publication

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

H2B-tdiRFP

Expression

Mammalian

Promoter

CAG

Available since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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