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We narrowed to 4,840 results for: phy

Showing: 781 - 800 of 4840 results

  1. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  6. pICH47802-p35S::tdTomato-ER::tNOS

    Plasmid
    #160220
    Purpose
    tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse
    Depositor
    Matias Kirst
    Article
    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.
    Insert
    p2x35S::TdTomato-ER::tNOS
    Use
    Synthetic Biology; Moclo toolkit level 1 acceptor…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    160220-plasmid-map-sequence-id-316582

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  7. pET22b_EcSSB_1

    Plasmid
    #78203
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1
    Insert
    ssb (G26C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G26C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78203-plasmid-map-sequence-id-151729

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  8. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  9. pZA8

    Plasmid
    #158486
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158486-plasmid-map-sequence-id-310749

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  11. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  12. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  13. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad family member 4 (SMAD4 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49383-plasmid-map-sequence-id-73406

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Showing: 781 - 800 of 4840 results