165,343 results

1. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mScarlet-EFS-Blast

   Plasmid

   #227260

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Ezrin-N-14

   Plasmid

   #55043

   Purpose

   Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Ezrin (EZR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. moxGFP

   Plasmid

   #68070

   Purpose

   C-terminal fusion to moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Type

   Empty backbone

   Tags

   moxGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemon in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Expression

   Bacterial

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT525

   Plasmid

   #131392

   Purpose

   L1RP retrotransposition reporter

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   L1RP GFP-AI
   

   Expression

   Mammalian

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 hMOG-alpha1-EGFP

   Plasmid

   #126463

   Purpose

   Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Myelin oligodendrocyte glycoprotein (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

    Plasmid

    #133027

    Purpose

    Expresses EGFP-tagged late endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB7A (RAB7A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKC eta DN

    Plasmid

    #21246

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K384R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHW449

    Plasmid

    #198805

    Purpose

    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Shigeo Sugano, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-∆Hapln1

    Plasmid

    #200778

    Purpose

    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Mutation

    deleted lectican binding domain (aminoacids 40-15…

    Promoter

    Synapsin

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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