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pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

Plasmid

#166834

Purpose

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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VEGFR2-mCh

Plasmid

#108854

Purpose

Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with mCherry on the C-Terminus via a 3 am…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

Plasmid

#227004

Purpose

AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

Insert

alpha synuclein A53T mutant (SNCA Human, Synthetic)

Use

AAV

Mutation

A53T

Promoter

CMVenh synapsin

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-C1-mCherry-LOVPLD

Plasmid

#219559

Purpose

ER-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

C1-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEN243-EN1953

Plasmid

#224546

Purpose

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEB1-2ndVal mGFPmut2

Plasmid

#104033

Purpose

Plasmid encoding 2ndVal mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

mGFPmut2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Tags

His8 + SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130989

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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anti-Jagged_D5-pBIOCAM5

Plasmid

#39345

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv specific for Jagged (clone D5) (JAG1 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDY118A

Plasmid

#182958

Purpose

pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Inserts

Cas9
Gam, Beta, Exo
I-scel

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Expression

Mammalian

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP2

Plasmid

#135551

Purpose

Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP2

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD40-Fc(DAPA)-AviTag-6xHis

Plasmid

#156572

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD40 (CD40 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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IO001: pMVP (R4-R3) eGFP

Plasmid

#121730

Purpose

pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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CRBN_ΔHBD

Plasmid

#232779

Purpose

Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

Depositor

Ivan Dikic

Article

Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

Insert

Cereblon (CRBN Human)

Tags

MBP-His6-TEV

Expression

Bacterial

Mutation

CRBN (residues 47 to 193 and 249 to 436) with GNG…

Promoter

T7

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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human syndecan 2 EFYA

Plasmid

#64971

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

deleted EFYA

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
human syndecan 2 FAAF

Plasmid

#64972

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

Y179F, S187A, S188A, Y191F

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21

Plasmid

#78782

Purpose

To overexpress p21 in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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