19,660 results

1. pMLRT

   Plasmid

   #78234

   Purpose

   Expresses Murine Leukemia Reverse Transcriptase (MLRT)

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Insert

   Murine leukemia reverse transcriptase
   

   Expression

   Bacterial

   Promoter

   J23102

   Available Since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS300

   Plasmid

   #22846

   Purpose

   Backbone for expressing plant artificial miRNAs

   Depositor

   Detlef Weigel

   Article

   Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

   Insert

   MIR319a (MIR319a Mustard Weed)
   

   Use

   RNAi; Plant gene inactivation

   Expression

   Plant

   Available Since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEx-4T-1-YTHDF2

   Plasmid

   #52301

   Purpose

   Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pME-puro-SNAP-FLAG-CD59

   Plasmid

   #50374

   Purpose

   Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   SNAP
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmScarlet-i_Giantin_C1

   Plasmid

   #85050

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-GB1

   Plasmid

   #112565

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-GB1 and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-myc3-bTrCP

   Plasmid

   #20718

   Depositor

   Yue Xiong

   Article

   Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

   Insert

   beta-transducin repeat containing (BTRC) (Btrc Mouse)
   

   Tags

   Myc3

   Expression

   Mammalian

   Mutation

   *

   Available Since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.3G_X7

   Plasmid

   #171213

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites

   Depositor

   Jenny Wang

   Insert

   N/A; empty backbone
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-ACE2

    Plasmid

    #141185

    Purpose

    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-BAZ2A

    Plasmid

    #65373

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ2A (BAZ2A Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH3U3-zif268 (omega)

    Plasmid

    #18046

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268 binding site
    

    Expression

    Bacterial

    Mutation

    The preferred binding site for zif268 is inserted…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

    Plasmid

    #129719

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mEGFP

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJC49

    Plasmid

    #133060

    Purpose

    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mScarletI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-P3-Alb-mNG

    Plasmid

    #183460

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 SARS-CoV-2 M

    Plasmid

    #158078

    Purpose

    Expresses SARS-CoV-2 membrane protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 matrix (M )
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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