Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,501 results for: ada

Showing: 8021 - 8040 of 10501 results

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PB-PARP1-Apex2–eGFP

Plasmid

#211555

Purpose

A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

Apex2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-SHLD1

Plasmid

#114115

Purpose

Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEHC_PGKneoLox2DTA.2

Plasmid

#112623

Purpose

H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Human, Aequorea victoria)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Emerald

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-deltaH-AMBRA1-3xFLAG

Plasmid

#174158

Purpose

Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 1-34

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-M85E-3XHA

Plasmid

#242416

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M85E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D M85E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E M85E M92E-3XHA

Plasmid

#242418

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with V82E, M85E, and M92E mutations and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E M85E M92E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E-3XHA

Plasmid

#242393

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a V82E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-M92E-3XHA

Plasmid

#242417

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M92E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D M92E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d10-52-3xHA (Δα1)

Plasmid

#232004

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the first alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ10-52

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-96-3xHA (Δα2)

Plasmid

#232005

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d97-106-3xHA (Δα2/3 loop)

Plasmid

#232006

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the loop connecting the second and third alpha helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ97-106

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

Plasmid

#232007

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ106-113

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

Plasmid

#232008

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ118-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-138-3xHA (Δα2-4)

Plasmid

#232009

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second through fourth helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d159-174-3xHA (Δα5)

Plasmid

#232010

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fifth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ159-174

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d201-213-HA (ΔVPS4 binding)

Plasmid

#232011

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the VPS4-binding region deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

HA

Expression

Mammalian

Mutation

L4D F5D Δ201-213

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-YFP-Rb-IRES-blasticidin

Plasmid

#223964

Purpose

Fluorescent reporter with exogenous Rb

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

Plasmid

#145839

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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CROPseq-MPSV-eBFP2

Plasmid

#220472

Purpose

Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

Depositor

Christian Krebs

Article

Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Mutation

E225A/D227A

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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