We narrowed to 54,790 results for: plasmid

1. pUdO2a

   Plasmid

   #210227

   Purpose

   medium-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4_HA-Halo-cmyc-GRM3 C127A

   Plasmid

   #140458

   Purpose

   Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   Halo, Signal/leader sequence from influenza hemag…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, Threonine 174 to alanine

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

   Plasmid

   #105635

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD44I-c011

   Plasmid

   #175449

   Purpose

   Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-Clover-HIF-1alpha S31D

   Plasmid

   #163370

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S31D

   Promoter

   CMV Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-Clover-HIF-1alpha S31G

   Plasmid

   #163369

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S31G

   Promoter

   CMV Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-Clover-HIF-1alpha S786D

   Plasmid

   #163368

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Asp) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S786D (HIF1A Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S786D

   Promoter

   CMV Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-Clover-HIF-1alpha S786A

   Plasmid

   #163367

   Purpose

   Express HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Ala) plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha S786A (HIF1A Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   S786A

   Promoter

   CMV Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4164342

   Plasmid

   #153553

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   accatg Kozak-START linker

   Expression

   Mammalian

   Mutation

   Gordon et al recoded

   Available Since

   June 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGBW-m4164341

    Plasmid

    #153552

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    accatg Kozak-START linker

    Expression

    Mammalian

    Mutation

    Gordon et al recoded

    Available Since

    June 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGBW-m4137385

    Plasmid

    #149542

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    wt;18-aa Ctrunc

    Promoter

    CMV

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pGBW-m4046565

    Plasmid

    #149097

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pJ211_WT_med

    Plasmid

    #128849

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ211_rha_WT_hi

    Plasmid

    #128853

    Purpose

    High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTHRB-donor

    Plasmid

    #112333

    Purpose

    CRISPR donor plasmid to tag human transcription factor THRB with GFP

    Depositor

    Kevin White

    Insert

    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    

    Use

    CRISPR

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZNF445-donor

    Plasmid

    #112332

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP

    Depositor

    Kevin White

    Insert

    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    

    Use

    CRISPR

    Mutation

    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZNF785-donor

    Plasmid

    #112317

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

    Depositor

    Kevin White

    Insert

    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    

    Use

    CRISPR

    Mutation

    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p240_LTJ_sgRNAFoxp3K276X

    Plasmid

    #82675

    Purpose

    sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 K276X
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p204_LTJ_sgRNACD45.2_R2

    Plasmid

    #82673

    Purpose

    sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p163_LTJ_sgRNACD90.1

    Plasmid

    #82677

    Purpose

    sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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