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pcDNA3.1/GS-DJ1-E64D-V5

Plasmid

#29344

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Glu64Asp

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-DIO-SEP-GluA1

Plasmid

#227235

Purpose

Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA1 in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

SEP-GluA1 (Gria1 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

Plasmid

#233080

Purpose

Expression vector for sgRNA-S1b and eCas12f1i

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-UCP1-Flag

Plasmid

#234983

Purpose

Expresses UCP1 with C-terminal Flag tag in human cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

UCP1 (Ucp1 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-iPEmax-Hygro

Plasmid

#214020

Purpose

inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PEmax

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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CCR8-DuET

Plasmid

#213204

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR8 (CCR8 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12123 - pAAV-AiE0397m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2123)

Plasmid

#208150

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AiP12102 - pAAV-AiE0387m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2102)

Plasmid

#208133

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD4

Plasmid

#195842

Purpose

Expresses human FZD4 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD4 (FZD4 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cluc-RMA

Plasmid

#189624

Purpose

Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

Cypridina luciferase fused to Fc

Use

AAV and Luciferase

Tags

Cluc and IgG1 Fc

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188774

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBFC0687

Plasmid

#186692

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR EGFR-SLP76-TagBFP

Plasmid

#188630

Purpose

Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Human, Synthetic)

Use

Lentiviral

Tags

SLP76-TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBsCas12a

Plasmid

#174671

Purpose

Expresses 6xHis-MBP tagged BsCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBsCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pM124: pAAV-EFS-CasRx-VEGFA presgRNA

Plasmid

#166872

Purpose

AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-VEGFA presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EFS and U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG1-T2A-PuroR

Plasmid

#162339

Purpose

Lentiviral expression of NEUROG1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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