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siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

Plasmid

#226578

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-BetaActin-SecA5-YFP

Plasmid

#105664

Purpose

Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

Depositor

Qing Deng

Article

Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

Insert

Secreted Annexin5-EYFP (ANXA5 Human)

Use

Tol2-gateway

Tags

EYFP

Promoter

Beta-Actin

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

Plasmid

#202822

Purpose

Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

two sgRNAs that delete exon 1 within the ZNF91 gene

Use

Lentiviral

Expression

Mammalian

Promoter

U6 - two

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318; A341)

Plasmid

#204194

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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MAVS-Mito

Plasmid

#44556

Purpose

Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS-Fis1 chimeric protein (MAVS Human)

Use

Retroviral

Expression

Mammalian

Mutation

contains aa1-500 of MAVS and aa127-152 of Fis1

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-ms-Klf5

Plasmid

#50787

Purpose

retroviral expression of mouse Klf5

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf5 (Klf5 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-BAFFR-CFP

Plasmid

#187002

Purpose

Expression of human BAFFR-CFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

BAFFR (TNFRSF13C Human)

Use

Lentiviral

Tags

CFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn LAMP1-msGFP (JV012)

Plasmid

#179728

Purpose

Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

LAMP1 (Lamp1 Mouse)

Use

Lentiviral

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

Plasmid

#187585

Purpose

Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NPAT (NPAT Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-RpHLuorin2

Plasmid

#171722

Purpose

Synchronize trafficking of TNFa-RpHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and ratiometri…

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-HMGA2

Plasmid

#52727

Purpose

retroviral expression of human HMGA2

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HMGA2 (HMGA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A45

Plasmid

#132054

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A45 (SLC25A45 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SV2C

Plasmid

#132297

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2C (SV2C Human)

Expression

Mammalian

Available Since

Nov. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-ColX

Plasmid

#222305

Purpose

Synchronize the trafficking of CollagenX from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)

Expression

Mammalian

Mutation

signal peptide of COL10A1 removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-gp135

Plasmid

#222306

Purpose

Synchronize the trafficking of gp135/podocalyxin from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 TIFA T9A

Plasmid

#231590

Purpose

TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

TIFA (TIFA Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

T9A

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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