19,662 results

1. p3990 pET ubcH8

   Plasmid

   #8650

   Depositor

   Peter Howley

   Article

   Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

   Insert

   UbcH8 (UBE2L6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Puro_siKO-2TO

   Plasmid

   #86697

   Purpose

   Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1 2TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LBR EGFP

   Plasmid

   #128150

   Purpose

   Expression of lamin B receptor

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

   Insert

   lamin B receptor (LBR Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

   Plasmid

   #164822

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   miniCMV and pGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp204-PADH1-atTIR1-9myc

   Plasmid

   #99532

   Purpose

   S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

   Depositor

   Helle Ulrich

   Insert

   TIR1 (TIR1 Mustard Weed)
   

   Tags

   9xMyc

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MLV Gag-RFP

   Plasmid

   #1814

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   Gag (gag MuLV)
   

   Tags

   RFP

   Expression

   Mammalian

   Available Since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-GFP-Sox11

   Plasmid

   #120387

   Purpose

   over expression of sox11 using infection

   Depositor

   Igor Ulitsky

   Article

   Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

   Insert

   sox11 (Sox11 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-GFP-AT

    Plasmid

    #133306

    Purpose

    "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    axe/txe toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ-CBE-Cas9n-Act3.0

    Plasmid

    #178955

    Purpose

    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM444

    Plasmid

    #108319

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHM830

    Plasmid

    #20702

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-MCS-LacZ
    

    Expression

    Mammalian

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFSp-GFP-Empty

    Plasmid

    #174171

    Purpose

    Control lentiviral vector expressing GFP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pVoPo-04

    Plasmid

    #187839

    Purpose

    Suicide vector to generate markerless gene deletions in Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJYDN

    Plasmid

    #162450

    Purpose

    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

    Expression

    Yeast

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Mouse, Synthetic)
    

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Promoter

    TET

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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