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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRRL-5xTetO-pEF-H2B-Citrine-SV40

Plasmid

#186966

Purpose

Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

5xTetO-pEF-H2B-Citrine-SV40

Use

Lentiviral

Expression

Mammalian

Promoter

5xTetO-pEF-1alpha

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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miR-34aM53

Plasmid

#50830

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M53 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

p53 binding site mutated (-39)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-hygro-PAX9

Plasmid

#75083

Purpose

[Edit] retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

full length, wild type, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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1199 pAdTrack FKHR AAA

Plasmid

#9037

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

Plasmid

#105201

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-P2A-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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miR-34aM2

Plasmid

#50828

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M2 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

second NF-kB binding site mutated (-511)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only
FUW-M2-PAK2 wt

Plasmid

#31680

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKmt2d-sg1

Plasmid

#192166

Purpose

Lenti-sgKmt2d-sg1

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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ZJOM156

Plasmid

#133636

Purpose

Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

SEC7-2GFP (SEC7 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPERIORpuro-shGAS5

Plasmid

#46370

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

RNAi

Promoter

H1

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
PKG I alpha dNPS

Plasmid

#16391

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: delet…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
PGL3-NOXA-N2

Plasmid

#26113

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

NOXA N2 (PMAIP1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 HA-rnSOX9 ΔC (1-381)

Plasmid

#62973

Purpose

Expresses HA-tagged rat SOX9 residues 1-383

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA

Expression

Mammalian

Mutation

codes for residues 1-381

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pEF Itk myc

Plasmid

#20705

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk (Itk Mouse)

Tags

myc

Expression

Mammalian

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
TRIN-shREN

Plasmid

#105586

Purpose

Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only
pLVX puro TRIM21 C54Y - GFP

Plasmid

#116942

Purpose

Expression of TRIM21 C54Y -GFP fusion

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 C54Y-GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

C54Y

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mIR-31 sponge

Plasmid

#22694

Purpose

Stable suppression of miR-31 function

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Tags

*

Expression

Mammalian

Mutation

miR-31 sponge with 7x tandem binding sites to the…

Available Since

March 11, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

Plasmid

#170467

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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