We narrowed to 65,929 results for: des.1

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pJA54

Plasmid

#60212

Purpose

cleavage near e1350 locus in sqt-1

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA1

Use

CRISPR

Promoter

U6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pJH4.66

Plasmid

#26951

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

beta-tubulin (tbb-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-Glutamate_Tag

Plasmid

#46367

Depositor

Mark Howarth

Article

Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

Insert

Streptavidin-Glutamate_Tag

Tags

6 glutamate tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only
pET21a-Core Traptavidin

Plasmid

#26054

Depositor

Mark Howarth

Article

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Core Traptavidin

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G R5…

Available Since

Aug. 30, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-Gfi1

Plasmid

#158531

Purpose

Expresses human Gfi1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

Growth Factor Independent Protein 1 (GFI1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP1

Plasmid

#75163

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIPk1

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIPK1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETB PKA RIalpha

Plasmid

#14922

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA R1alpha regulatory subunit (PRKAR1A Bovine)

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pmRFPmars

Plasmid

#26252

Depositor

Martin Fraunholz

Article

Paprotka et al J Microbiol Methods. 2010 Oct;83(1):82-6

Insert

mRFPmars

Tags

6xHis

Expression

Bacterial

Mutation

KpnI restriction site removed

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
Flag-ECFP-Paxillin

Plasmid

#15211

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

paxillin (PXN Chicken)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
Pmec-18 ced-3 (p15)::nz [TU#806]

Plasmid

#16080

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

ced-3 (p15) :: nz (ced-3 Nematode)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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Pmec-18 cz::ced-3 (p17) [TU#807]

Plasmid

#16081

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz:: ced-3 (p17) (ced-3 Nematode)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Tags

Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Kir6.1 K+ channel scFv [N366/60]

Plasmid

#190547

Purpose

Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GST-FAST-FH

Plasmid

#16524

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

FAST-1 (FH domain) (FOXH1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-150. Forkhead domain.

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pACYC-T7

Plasmid

#41187

Depositor

Dan Bolon

Article

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRSII413

Plasmid

#35450

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-Apex2–eGFP

Plasmid

#211555

Purpose

A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

Apex2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG227-Wnt1

Plasmid

#99073

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Wnt1 (also known as WntP-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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