We narrowed to 7,354 results for: Ank

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LUC7L

Plasmid

#155713

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

LUC7L (LUC7L Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1097

Plasmid

#29028

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Moe-pDONR221

Plasmid

#124239

Purpose

Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Insert

Drosophila Moesin (Moe Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCAD108_Arg273His_pCMV6Entry-hIRG1

Plasmid

#124878

Purpose

Expresses hCAD Arg273His mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Arg273 to His

Promoter

CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_AE

Plasmid

#148577

Purpose

Mammalian Expression of HsGIGYF2

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

Plasmid

#79889

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Expression

Bacterial

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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